Autor: Agata Szczepanik

nitroxides

The role of nitroxides in EPR imaging

EPR is making its way to clinical studies Nowadays neurodegenerative disease such as Parkinson and Alzheimer’s are well known from taking their tolls. It is worth noticing that the imbalance between antioxidants and reactive oxygen species leads to damage caused by oxidative stress, and the oxidative damage is known for having an impact on the development of these diseases. The use of non-invasive EPR scanners and blood-brain permeative nitroxides, e.g. HMP or MCP, may be very helpful in the monitoring of changes in redox status. The Japanese research group led by M.C Emoto, has carried out an examination of the distribution and time courses reduction of 3-hydroxymethyl-2,2,5,5-tetramethylpyrrolidine-1-oxyl (HMP) and 3-methoxycarbonyl-2,2,5,5-tetramethylpyrrolidine-1-yloxy (MCP). Studies were presented in the paper “Dynamic changes in the distribution and time course of blood-brain barrier-permeative nitroxides in the mouse head with EPR imaging: visualization of blood flow in a mouse model of ischemia”. The process of examination have consisted of several stages, which included the anatomical imaging of an animal by MRI, the injection of MCP and HMP into the mouse, and the non-invasive imaging of the brain by a 3D EPR tomograph. After the nitroxides were injected, the changes in redox state in brains of the mice were monitored. As a result, very significant conclusions have been reached. Co-registered imaging clearly pointed out the differences in localization of both nitroxides. HMP has been distributed uniformly in mouse head and, contrary to MCP, has not been accumulated within the brain. The difference has been also found in perfusion rate, for HMP seems to be slower. After the in vivo studies, the mice were euthanized in order to assess the effect of blood flow on the redox reaction. Without blood flow, the half-life of HMP was significantly longer, while the half-life of MCP did not change at all. Nevertheless, MCP seems to be reduced inside  the brain with no loss of MCP from the brain by washout. It has been previously reported that many hydrophilic and lipophilic nitroxide compounds provide significant physiological information while being used as imaging probes. Designing of the experiment and the selection of the appropriate nitroxide spin probe can provide unique biological information, but it needs to be done with caution due to the lack of information about details concerning the time-dependent distribution and in vivo pharmacokinetics for the examination of small animals. References: Emoto MC, Sato-Akaba, Hirata, Fujii HG; Dynamic changes in the distribution and time course of blood-brain barrier-permeative nitroxides in the mouse head with EPR imaging: visualization of blood flow in a mouse model of ischemia; Free Radic Biol Med. 2014 Sep;74:222-8; DOI: 10.1016/j.freeradbiomed.2014.06.026.

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mouse epr

The Bright Side of Electron Paramagnetic Resonance.

The Bright Side of Electron Paramagnetic Resonance. Monitoring the balance between oxidants and anti-oxidants, which is defined as a redox status, may help with providing a more efficient treatment of tumours, but it may also allow for a better understanding of neurodegenerative diseases. The balance between oxidation and reduction plays a crucial role in the well-being of lives. Electron Paramagnetic Resonance is a unique and non-invasive imaging tool which is capable of detecting redox-sensitive probes which can map the redox environment of a tissue or a cell. A research group from the Ohio State University has collected the advantages and examples of the use of EPR imaging and presented them in a paper titled “Redox Mapping of Biological Samples Using EPR Imaging”. Oxidative stress has been found to be one of the indicators of many pathological changes such as malignancy or inflammation. The possibility of acquiring a redox map of the tumour, provided by EPR imaging, can lead to the development of more effective therapeutic strategies. The authors of the paper presented a few experiments that showed the potential of EPR imaging. In the first one, the process of creating a redox map using electron paramagnetic resonance was described. Then the authors presented examples of application of imaging redox status in malignant tissue versus a normal one, in radiation-induced fibrosarcoma. There is also an examination of the effect of UV exposure on skin redox status. In another studies, imaging of the redox state was used to estimate the in vivo intracerebral reducing ability of mature rats after neonatal hypoxic-ischemic brain injury and to show the ischemic regions in isolated rat’s hearts. The last example of the use of EPR imaging concerned the use of a nitroxide spin label to measure the oxidative stress in the brain with Alzheimer’s disease. It has been confirmed that EPR imaging has a wide range of applications and it is found to be helpful for the detection and the examination of not only oncological diseases, but also other pathological states in living organisms. References: Aditi C. Kulkarni, Anna Bratasz, Brian Rivera, Murali C. Krishna, Periannan Kuppusamy; Redox Mapping of Biological Samples Using EPR Imaging; Israel Journal of Chemistry (Online) 48(1):27 – 31; DOI: 10.1560/IJC.48.1.27.

The Bright Side of Electron Paramagnetic Resonance. Dowiedz się więcej »

antioxidans in vegetables

5 servings of fruit and vegetables – why are they so important?

5 servings of fruit and vegetables – why are they so important? “You should have at least 5 portions of fruit and vegetables every day” Sounds familiar? I bet you said yes. Doctors and nutritionists around the world constantly remind us of the importance of eating greens. See what we discovered and see why. Fruit and vegetables apart from all the beneficial vitamins and nutrients are important sources of antioxidants. Antioxidants slow down the ageing process and protect against the harmful effect of free radicals. It is best to eat raw fruit and vegetables and juice may be the perfect option on a busy day. We decided to conduct a simple experiment in which we compared peach and carrot juice. We wanted to see how they handle free radicals scavenging. We filled 0.5 ml Eppendorf with 150 ml of carrot juice and 150 ml of 1 mM nitroxide spin probe. The EPR signal from the sample was measured for 15 minutes using the Novilet L-band system. Then an analogous experiment was carried out with peach juice. After 15 minutes of the experiment, the signal from the carrot juice decreased exponentially by nearly 80%, while in the case of peach juice, the signal decay was linear and only decreased by about 20% So what did we conclude? The results showed that carrot juice was significantly more efficient at scavenging free radicals compared to peach juice. Quote to remember! The more juices you drink, the fewer free radicals you have! Cheers!

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antioxidants

Why you shouldn’t forget about your coffee?

Why you shouldn’t forget about your coffee, but drink it as fast as you can? Coffee is one of the most popular hot beverages consumed every day all over the world. For some people drinking coffee is like a ritual, for some, it is a way of boosting the energy throughout the day and for others, it is a perfect choice during the first date. It is not surprising because who doesn’t like the scent of freshly brewed coffee in the morning? The debate on the impact of coffee on the human organism is going on for a while now. In an appropriate dose, coffee can be considered a beneficial source of antioxidants. We decided that, since we work so much with free radicals we could show you the information we learned after we looked more closely inside our cups. How many times did you make yourself a coffee at work or at home and then forget about it? We wanted to see if the antioxidant properties of coffee change after some time, and when it is the best time to drink it. We performed a simple experiment with our L-Band spectrometer. Easy access to the measured area, as well as straight-froward sample preparation, let us do the experiment during… well… a coffee break (who would expect that, right? ?). We compared the antioxidant capacity of the freshly brewed coffee with the coffee 1 hour after brewing. It appears that freshly brewed coffee has about 30% better antioxidant properties than the beverage that was tested later. We can’t wait to see other results. We think that comparing different types of coffee would be very interesting, to see how the brewing temperature can affect the antioxidant properties, how the antioxidant capacity impacts the taste. If you would be interested in carrying out those types of experiments let us know and we will discover the secrets of coffee together! Cheers! ☕☕☕

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clinical_epr

EPR is making its way to clinical studies

EPR is making its way to clinical studies It was recently reported by Schaner PE et. all that the Electron Paramagnetic Resonance technique together with a specially designed spin probe called OxyChip can be successfully applied in clinical trials. This is the first reported example of this new approach. The method has the potential to complete the diagnostic and monitoring of the development of several types of cancers. 23 patients with severe cancer were enrolled in the study. OxyChips were implanted inside tumours and then the data was collected for a certain period of time. The signals were successfully obtained in 16 patients. The data directly showed the oxygen concentration in tumours, which allowed the authors to clarify if the cancer is hypoxic. The signals were collected by the surface resonator placed on the skin, where the OxoChip was implanted. In a conclusion, with the combination of EPR with OxoChip, it is possible to measure the level of oxygen in tumours directly from the patient’s body. The undoubted advantage of this method is repeatable and quick data collection. The uniqueness of the technique is the ability to repeat the measurements many times without the patient’s discomfort. References: Philip E. Schaner et.al.: First-In-Human Study in Cancer Patients Establishing the Feasibility of Oxygen Measurements in Tumors Using Electron Paramagnetic Resonance With the OxyChip, Front. Oncol. 2021 Oct 1;11:743256.

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Zapytanie ofertowe nr 28/POiR.01.02.00-0077/18-00 z dnia 11 lutego 2022 r.

Zapytanie ofertowe nr 28/POiR.01.02.00-0077/18-00 z dnia 11 lutego 2022 r. Przedmiotem zamówienia jest: „Zakup silników” w ramach projektu nr POIR.01.02.00-00-0077/18-00 o tytule: „Innowacyjny tomograf impulsowy do przestrzennego obrazowania zmian neurodegeneracyjnych z wykorzystaniem techniki rezonansu elektronowego” współfinansowanego ze środków Unii Europejskiej w ramach Programu Operacyjnego Inteligentny Rozwój, działanie 1.2., sektorowe programy B+R InnoNeuroPharm. Zapytanie ofertowe nr 26/POiR.01.02.00-0077/18-00 z dnia 11 lutego 2022 r..pdf Załącznik nr 1.pdf Załącznik nr 2.xls Załącznik nr 3.pdf

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Zapytanie ofertowe nr 27/POIR.01.02.00-00-0077/18-00 z dnia 11 lutego 2022 r.

Zapytanie ofertowe nr 27/POIR.01.02.00-00-0077/18-00 z dnia 11 lutego 2022 r. Przedmiotem zamówienia jest: „Zakup części ogólnego zastosowania 8” w ramach projektu nr POIR.01.02.00-00-0077/18-00 o tytule: „Innowacyjny tomograf impulsowy do przestrzennego obrazowania zmian neurodegeneracyjnych z wykorzystaniem techniki rezonansu elektronowego” współfinansowanego ze środków Unii Europejskiej w ramach Programu Operacyjnego Inteligentny Rozwój, działanie 1.2., sektorowe programy B+R InnoNeuroPharm. Zapytanie ofertowe nr 27/POIR.01.02.00-00-0077/18-00 z dnia 01 grudnia 2021 r.pdf Załącznik nr 1.pdf Załącznik nr 2.xls Załącznik nr 3. pdf 

Zapytanie ofertowe nr 27/POIR.01.02.00-00-0077/18-00 z dnia 11 lutego 2022 r. Dowiedz się więcej »

Zapytanie ofertowe nr 26/POiR.01.02.00-0077/18-00 z dnia 17 stycznia 2022 r.

Zapytanie ofertowe nr 26/POiR.01.02.00-0077/18-00 z dnia 17 stycznia 2022 r. Przedmiotem zamówienia jest: „Zakup części z tworzywa sztucznego 6” w ramach projektu nr POIR.01.02.00-00-0077/18-00 o tytule: „Innowacyjny tomograf impulsowy do przestrzennego obrazowania zmian neurodegeneracyjnych z wykorzystaniem techniki rezonansu elektronowego” współfinansowanego ze środków Unii Europejskiej w ramach Programu Operacyjnego Inteligentny Rozwój, działanie 1.2., sektorowe programy B+R InnoNeuroPharm. Zapytanie ofertowe nr 26/POiR.01.02.00-0077/18-00 z dnia 17 stycznia 2022 r. pdf Załącznik nr1. pdf Załącznik nr 2.xls Załącznik nr 3.pdf

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Zapytanie ofertowe nr 25/POiR.01.02.00-0077/18-00 z dnia 10 grudnia 2021r.

Zapytanie ofertowe nr 25/POiR.01.02.00-0077/18-00 z dnia 10 grudnia 2021r. Przedmiotem zamówienia jest: „Zakup części z tworzywa sztucznego 5” w ramach projektu nr POIR.01.02.00-00-0077/18-00 o tytule: „Innowacyjny tomograf impulsowy do przestrzennego obrazowania zmian neurodegeneracyjnych z wykorzystaniem techniki rezonansu elektronowego” współfinansowanego ze środków Unii Europejskiej w ramach Programu Operacyjnego Inteligentny Rozwój, działanie 1.2., sektorowe programy B+R InnoNeuroPharm.  Zapytanie ofertowe nr 25/POiR.01.02.00-0077/18-00 z dnia 10 grudnia 2021r.pdf Załącznik nr1. pdf Załącznik nr 2.xls Załącznik nr 3.pdf

Zapytanie ofertowe nr 25/POiR.01.02.00-0077/18-00 z dnia 10 grudnia 2021r. Dowiedz się więcej »

Zapytanie ofertowe nr 24/POIR.01.02.00-00-0077/18-00 z dnia 01 grudnia 2021 r.

Zapytanie ofertowe nr 24/POIR.01.02.00-00-0077/18-00 z dnia 01 grudnia 2021 r. Przedmiotem zamówienia jest: „Zakup części maszyn ogólnego zastosowania 7” w ramach projektu nr POIR.01.02.00-00-0077/18-00 o tytule: „Innowacyjny tomograf impulsowy do przestrzennego obrazowania zmian neurodegeneracyjnych z wykorzystaniem techniki rezonansu elektronowego” współfinansowanego ze środków Unii Europejskiej w ramach Programu Operacyjnego Inteligentny Rozwój, działanie 1.2., sektorowe programy B+R InnoNeuroPharm. Zapytanie ofertowe nr 24/POIR.01.02.00-00-0077/18-00 z dnia 01 grudnia 2021 r.pdf Załącznik nr 1.pdf Załącznik nr 2.xls Załącznik nr 3. pdf 

Zapytanie ofertowe nr 24/POIR.01.02.00-00-0077/18-00 z dnia 01 grudnia 2021 r. Dowiedz się więcej »

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